Bioanalyzer Submissions

PLEASE NOTE: Due to continuing concerns posed by potential bioterrorism agents (select agents), our online submission form requires that you provide us with information on the original biological source of any DNA from which you will be asking us to obtain sequence. "Original biological source" refers to the organism from which the DNA was first found in nature (human, HIV, Staphylococcus aureus, etc.). If you simply put a nonspecific term such as "E. coli clone", this will not be sufficient. We realize that adding yet another field to fill out on these forms can be tiresome, but this information is necessary to protect our staff against potential legal difficulties. Thank you very much for your understanding.

           

 

 Bioanalyzer Submissions

Preparation of samples:

1. Please submit a minimum of at least 3ul of each sample in an RNase/DNase free 0.6ml eppendorf tube.


2. Dilute RNA to the following recommended sample concentrations:

   Total RNA and mRNA	25-500 ng/ul
   Small RNA (6-150 nt)	1-100 ng/ul (Total RNA)
   	1-20 ng/ul (Enriched small RNA)
   	100-2000 ng/ul (Oligonucleotides)



3. A submission form must be filled out completely and submitted with your samples.

4. An SUR# (OUHSC) or PO# (off-campus) must be given at the time of submission.
   
   Shipping Address:
   
   The Laboratory for Genomics and Bioinformatics OUHSC
   Attn: Microgen Support
   975 NE 10th Street, BRC1106
   Oklahoma City, OK 73104
   

6. Samples will be processed within 24hrs and results e-mailed as a .pdf. If you wish to do further analysis such as overlaying multiple samples and comparisons between chips please indicate you want to receive the .xad experimental files on the order form, then download the latest Bioanalyzer B.02.05 software.Agilent Bioanalyzer software download instructions.


7. Pricing is $35.00 per chip. The chips are one use (empty wells can not be rerun). The RNA 6000 Nano chip for analysis of total RNA and mRNA accommodates up to 12 samples. The Small RNA chip has 11 wells for experimental samples.


8. Place samples in the freezer located in BRC room 1106