PLEASE NOTE: Due to continuing concerns posed by potential bioterrorism agents (select agents), our online submission form requires that you provide us with information on the original biological source of any DNA from which you will be asking us to obtain sequence. "Original biological source" refers to the organism from which the DNA was first found in nature (human, HIV, Staphylococcus aureus, etc.). If you simply put a nonspecific term such as "E. coli clone", this will not be sufficient. We realize that adding yet another field to fill out on these forms can be tiresome, but this information is necessary to protect our staff against potential legal difficulties. Thank you very much for your understanding.

           

 

 Sequencing Submission and Instructions

Important notes:

• We now offer plasmid growth, plasmid prep (Qiagen QIAprep Spin columns), and PCR clean-up (Qiagen columns).


• All samples should be placed in the white freezer in BRC Room 1106 and an online Sequencing Submission Form (link included on appropriate instruction form) must be completed before samples will be processed.


• We now accept any number of samples in microfuge tubes but will give a discount to customers who submit their templates with primers pre-mixed in the appropriate amounts as described in the instructions.


• Although data turn-around times are listed in the specific instructions below, please keep in mind that times may be slightly delayed by high volumes of submission, weekends, or holidays.


• The facility provides the following Universal Primers free of charge:
  
  
  • M13F: 5'-TGT AAA ACG ACG GCC AGT-3'
  
  
  • M13R: 5'-CAG GAA ACA GCT ATG AC-3'
  
  
  • T7: 5'-TAA TAC GAC TCA CTA TAG GG-3'
  
  
  • T7: Term 5'-GCT AGT TAT TGG TCA GCG G-3'
  
  
  • T3: 5'-ATT AAC CCT CAC TAA AG-3'
  
  
  • SP6: 5'-GAT TTA GGT GAC ACT ATA G-3'
  
  
  • SK: 5'-CGC TCT AGA ACT AGT GGA TC-3'
  
  
  • KS: 5'-TCG AGG TCG ACG GTA TC-3'
  
  
  • PGEX3': 5'-CCG GGA GCT GCA TGT GTC AGA GG-3'
  
  
  • PGEX5': 5'-GGG CTG GCA AGC CAC GTT TGG TG-3'

Links to sample submission instructions:

• Submitting cell pellets for plasmid prep and sequencing


• Submission of an existing plasmid prep


• Submission of PCR products for clean-up and sequencing


• Submission of PCR products for primer addition and sequencing


• Submitting template and primer already combined for sequencing


• Instructions and address for shipping samples