Submitting template and primer already combined for sequencing:

1. Please use a maximum of 4µl volume containing template (plasmid=200-300ng total or PCR product=10ng/100bps of product) plus primer (3.2pmol final concentration) per tube or well.


2. Note that 500-600ng of DNA is suggested for templates around the size of pUC (~2.9kb). More DNA might be needed for larger templates.


3. You can submit these samples frozen or dried down. If they are not dried down, please be sure to seal them properly so that evaporation does not occur.


4. Fill out the on line Sequencing Submission Form.

If samples are submitted by 3pm, you can expect your data back within 48 hours. If you submit by 3pm on Monday, you will receive data by 5pm on Wednesday. Samples submitted by Friday will be processed beginning the following Monday.

Other Notes:

1. Label template tubes with only the numerical designation (i.e. 1, 2, 3, 4, 5, 6, or 7) that corresponds to the appropriate sample information on the online submission form.
   
   
   • If you do not have access to a V-bottom or PCR plate at the time of submission but would like to use this method instead we will kindly provide you with a plate for your first submission FREE of charge.


2. If you are submitting <96 samples, then load the plate in column format (i.e. A1-H1, A2-H2 etc.)


3. Partial plates can be reused for submission, in which case you can use the first available column (i.e. starting with A4-H4 etc.) Just be sure that if you start with A4 on your plate you also begin with A4 on the submission form.
   
   
   • If you submit plates containing gaps between samples within a column you will be charged the regular sequencing rate for these gaps.
   
   
   
   • Finished sequences will be named as follows:
     
     
     • date of run+run#_well#_sample#_capillary#.filetype
     • example: 2002062101_A01_a01_001.ab1
     • - - [note: the date is set up as (YYYY/MM/DD)]
     • - - [note: the well# has capital letters while sample# has lower case letters]
     
     
     
   • Be sure to pay attention to the sample# since that number will correspond to the well position in which you submitted your sample. The well# on the finished sequence name will not always be the same as the sample#.


4. For a full plate of reactions, we typically see an efficiency of 80% or better. This efficiency will vary, however, with different templates. Efficiency is expected to drop with more difficult templates, such as : AT or GC rich templates.



5. Once the samples have been sequenced, leftover templates and primers should be picked up within 2 weeks.



6. If you would like to pick up partial plates for re-use, please indicate in the special instructions of your order that you would like your plate saved for pickup. Remember that when plates are submitted with only certain parts of the plate covered, water may collect in uncovered wells during freezer storage. Reuse those plates at your own risk. We ask that people only take back plates that were submitted by them. We cannot be held responsible for lost or stolen plates. If you have any questions, please feel free to contact microgen_support@ouhsc.edu.

 

 SUBMISSION FORMS

             online submission form   (for single plate submissions)

             high-throughput submission form   (for multiple-plate submissions)